Crushing Leaf In Eppendorf Using Vortex

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Methods to Transfer Foreign Genes to Plants | IntechOpen

Cut each plant leaf disc using a cork borer (3–4 mm diameter) or disposable blade (leaf piece should be approximately 3 mm × 3 mm). Place the leaf disc into an Eppendorf tube. Add 100 µl of Buffer A. Incubate for 10 min at 95 ºC. Vortex thoroughly. Transfer 0.5 µl of the template DNA supernatant to a PCR tube. PCR detection method. Reagents

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Transfer the supernatant (~600 ul) to a phase lock tube (can pour the supernatant, don't worry about floating leaf pieces). Add 600 ul phenol-chloroform and mix by inversion. (Caution: do not vortex Phase Lock tubes) 5. Spin tubes for 5 minutes at 12,000 RPM, pour off supernatant into a clean eppendorf …

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In planta Transcriptome Analysis of Pseudomonas syringae

Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv. tomato DC3000, in the leaves of Arabidopsis thaliana at an early stage of infection using RNA sequencing (RNA-Seq).

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An efficient protocol for DNA extraction from leaves of ...

An efficient protocol for DNA extraction from leaves of woody plants? ... were placed in a centrifuge tube type Eppendorf 1.5 ml containing 500 ml of extraction buffer (T = 65 ° C) of the ...

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- Easy-to-use Touch On operation. - Large, 6mm orbit for aggressive vortexing of difficult samples. - May be used with Pop-off Cup (shown) or optional 3-inch platform. - May be used in cold rooms and incubators. - Supplied with cup head for a single tube.

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Extraction of Genomic DNA from Rice Leaf by using a 5-ml ...

Extraction of Genomic DNA from Rice Leaf by using a 5-ml Tube ... Crush 0.3 g of rice leaf, put it into the 5-ml tube, turn it upside down, and then mix it by ... Dispense 100 μl of TE buffer and completely dissolve it by using a vortex. (25) Pour the mixture into a 1.5-ml tube and store it in a freezer.

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What's the best way to crush plant leaves for genomic DNA ...

What's the best way to crush plant leaves for genomic DNA isolation using Qiagen DNeasy kit? ... The best way is not using kit.Crush the leaves after washing with buffer using mortar and pestle ...

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DNA isolation from herbarium leaf tissue using DNeasy ...

DNA isolation from herbarium leaf tissue using DNeasy Plant Mini Kit ... Place ca. 20 mg of herbarium leaf tissues into a 2 ml safe lock (Eppendorf ... with disrupted leaf tissue and vortex vigorously using vortex mixer. Carry out this step in a fume hood 4) Incubate the mixtures in …

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